ATC L and CAF carried out the experiments on low dose of BtE. The mice were painlessly killed 24 h after the last allergen challenge. Eur J Immunol 2007, 37:1170–1173. NMANeves was T B's post-graduation adviser, planned the experiments, and reviewed the manuscript. Geographical distribution See common geographical background to mites in our Scientific Documents (link to the right). Total IgE was higher in BtE-sensitized animals (Figure 4A; p < 0001) and specific-IgE, IgG2a and IgG1 antibodies were higher in OVA-sensitized group (Figure 4B-D; p < 0.05, Tukey's test). PBS containing 1% of bovine serum albumin. On the other hand, OVA sensitization led to higher mucus production, and serum levels of specific IgE, IgG1 and IgG2a than BtE sensitization. Lab Anim Sci 1998,48(4):346–52. Immunization of A/J mice with BtE induced a more intense airway eosinophil influx, higher levels of total IgE, similar airway hyperreactivity to methacholine but less intense mucous production, and lower levels of specific IgE, IgG1 and IgG2 antibodies than sensitization with OVA. A significantly larger number of cells was found in the BALF of the mice sensitized with low-dose of BtE than in the BALF of the saline control group (Figure 5A; p < 0.01; Student's t test). Storage mites are a possible cause of allergic disorders and mostly present among agriculture population. Effect of immunization with 10 μg per injection ofB. The differences in IgE titers in BtE-sensitized and challenged mice in the four studied mouse strains were not statistically significant (p > 0.05; ANOVA), although A/J mice showed the highest titers, followed by the CBA/J, C57Bl/6 and BALB/c mice. © 2021 BioMed Central Ltd unless otherwise stated. tropicalis allergic responses in different mouse strains using a short-term model of respiratory allergy to BtE. (C) Anti-Bt or anti-OVA IgG2a antibodies. Clin Exp Allergy 1993,23(4):292–7. Farraj AK, Harkema JR, Jan TR, Kaminski NE: Immune responses in the lung and local lymph node of A/J mice to intranasal sensitization and challenge with adjuvant-free ovalbumin. Although a mouse model for B. tropicalis extract (Bt E)-induced asthma has been described, no study comparing different mouse strains in this asthma model has been reported. Sabit M, Ramos JDA, Alejandro GJ, and Gala C. Seasonal distribution of airborne pollen in Manila, Philippines, and the effect of meteorological factors to its daily concentrations.Aerobiologia 2015 (DOI 10.1007/s10453-015-9414-2). P > 0.05 is not represented. Am J Respir Crit Care Med 1999,160(4):1150–6. Descriere. Since A/J mice had more intense allergic responses, we selected this strain to make a comparison between the BtE-induced asthma model with the classical OVA-induced asthma model. Article  Blomia tropicalis (d201) IgE - This allergen-specific IgE antibody test quantifies an individual’s IgE response to Blomia tropicalis. Cite this article. volume 11, Article number: 51 (2010) The cell pellets were resuspended in PBS and lysed by three successive freezing and/thawing procedures, and then assayed for peroxidase activity in 96-well microassay plates, in duplicates, using 6.6 mM H2O2 and 1.5 mM orthophenylenodiamine (Merck, Whitehouse Station, NJ, USA). It may also infest stored food. Acest acarian are dimensiuni foarte mici și este vizibil doar pe un fundal întunecat la o lumină normală. The amount BtE used in the experiments was standardized by measuring its content in B. tropicalis Blo t 5 allergen, measured by a commercially available capture ELISA kit (INDOOR Biotechnologies, Charlottesville, VI, USA). 室塵 蟎 ( ㄇㄢˊ ) 屬(学名: Dermatophagoides )(英語: house dust mite )是一種8隻腳的微小動物,屬於節肢動物門 蛛形綱 恙蟎目 塵蟎科。 是台灣兒童主要的過敏原之一 。. Animals sensitized and challenged with BtE showed higher levels of total cells and eosinophils in the BALF than control mice (p < 0.001, Figure 2A, and p < 0.01, Figure 2B; Tukey's test). Its use as an assay for eosinophil differentiation factors. B. tropicalis house dust mites were collected from bed dust in Salvador, Brazil, cloned and cultured with a powdered fish food medium (Spirulina, Alcon Gold, São Paulo, Brazil), and dry yeast (Fermipan, São Paulo, Brazil), at 25°C and 75% humidity. de Brito CA, Fusaro AE, Victor JR, Rigato PO, Goldoni AG, Muniz BP, Duarte AJS, Sato MN, Bruno : CpG-Induced Th1-Type Response in the Downmodulation of Early Development of Allergy and Inhibition of B7 Expression on T Cells of Newborn Mice. State of the art Sensitization to this mite is very common in South America and Southeast Asia. PubMed  However, results obtained in murine experimental models of respiratory allergy that use OVA as antigen differ from those obtained in experimental models using mite allergens. Clin Exp Allergy 2002,32(11):1667–74. Hasegawa K, Tamari M, Shao C, Shimizu M, Takahashi N, Mao XQ, Yamasaki A, Kamada F, Doi S, Fujiwara H, Miyatake A, Fujita K, Tamura G, Matsubara Y, Shirakawa T, Suzuki Y: Variations in the C3, C3a receptor, and C5 genes affect susceptibility to bronchial asthma in human beings. Springer Nature. Mice were deeply anesthetized by intraperitoneal injection with a solution containing ketamine (Ketamina Agener; União Química Farmacêutica Nacional S/A, São Paulo, Brazil) and chloral hydrate (Labsynth, São Paulo, Brazil) and blood samples from the retro orbital plexus were collected for serum antibody level determinations. Data from A, B, and C are representative of three experiments, and from D of two experiments. MR contributed in planning the experiments and reviewing the manuscript. A/J mice were also considered the best responders to Dermatophagoides sp allergens among four studied strains [25]. Lowry OH, Rosenbrough NJ, Faar AL, Randall RJ: Protein measurement with the folin phenol reagent. (D) Bt-sensitized group. Tissues sections of 5-μm were then stained with periodic acid-Schiff (PAS) for the evaluation of mucus production. (C) Level of eosinophil peroxidase (EPO) activity in BALF. tropicalis IgG1 antibody levels in blood. 6. IFN-γ and IL-4, IL-10 and IL-13 concentration measurements were assayed in commercial ELISA kits according to manufacturer's instructions (Pharmingen, St. Diego, CA, USA). (D) Anti-B. EPO activity levels in BALF increased in all Bt-sensitized and challenged mice and was higher in A/J, CBA/J and C57Bl/6 mice than in BALB/c mice (p < 0.0001, p < 0.001, and p < 0.01, respectively; Tukey's test; Figure 1C). Dermatophagoides é um género de ácaros da família Pyroglyphidae presente na poeira intradomiciliar em todo o mundo. [11]. Strath M, Warren DJ, Sanderson CJ: Detection of eosinophils using an eosinophil peroxidase assay. Our mission is to enable our customers to make the world healthier, cleaner, and safer. By using this website, you agree to our Karp CL, Grupe A, Schadt E, Ewart SL, Keane-Moore M, Cuomo PJ, KöhI J, Wahl L, Kuperman D, Germer S, Aud D, Peltz G, Wills-Karp M: Identification of complement factor 5 as a susceptibility locus for experimental allergic asthma. Ramos JD, Cheong N, Lee BW, Chua KY: cDNA cloning and expression of Blo t 11, the Blomia tropicalis allergen homologous to paramyosin. Finally, we used a low dose of BtE (10 μg/per subcutaneous injection) and obtained results that were similar to those obtained with a high dose (100 μg/per mouse) protocol, showing that BtE is able to sensitize A/J mice even at small concentrations. Fernvik E, Peltre G, Sénéchal H, Vargaftig BB: Effects of birch pollen and traffic particulate matter on Th2 cytokines, immunoglobulin E levels and bronchial hyper-responsiveness in mice. CAS  Total cell counts were carried out using a Neubauer chamber., Atribuição-CompartilhaIgual 3.0 Não Adaptada (CC BY-SA 3.0) da Creative Commons. After the BALF collection, the lungs were perfused, via the heart right ventricle, to remove residual blood, immersed in 10% phosphate-buffered formalin for 24 h, followed by 70% ethanol, and embedded in paraffin. Using a short time model, we showed that A/J mouse strain was the best responder in terms of providing an experimental model of respiratory allergy. Respir Res 11, 51 (2010). It responded to immunization with the highest numbers of leukocytes in the BALF, consisting mainly of eosinophils, and had high levels of EPO activity in the BALF. Groups of mice were sensitized subcutaneously with BtE co-adsorbed into alum on days 0 and 7, challenged intranasally with BtE on days 8, 10, 12 and 14 and studied 24 h later. Sensitization with OVA (p < 0.001, Tukey's test) and BtE (p < 0.01, Tukey's test) induced AHR, as compared with control mice (Figure 2D). Immune response of BALB/c, C57Bl/6, CBA/J and A/J mice sensitized withBlomia tropicalisextract (closed symbols) or injected with saline (open symbols). Thus, experimental asthma models using common allergens might be more relevant tools to the study of human asthma [13]. Int Arch Allergy Immunol 2003,132(4):346–54. Health issues Allergies . High frequencies of positivity to B. tropicalis antigens in skin prick tests have been described in asthma and rhinitis patients, such as 68.1% in Cuba [7], 91.6% in Venezuela [8], 73.3% in Taiwan [9] and 95.0% in São Paulo, Brazil [10]. (A) Mucus Index in the bronchi. *p < 0.05, **p < 0.01, and ***p < 0.001 for the indicated tested groups (Tukey's test). J Investig Allergol Clin Immunol 1997,7(4):242–8. A quantitative digital morphometric analysis was performed using the application program Metamorph 6.0 (Universal Images Corp. Downingtown, PA, USA). CAS  Control groups received only alum and were challenged with saline or with BtE. It is abbreviated Blot by the World Health Organization. the time integral of changes in airway resistance within 20 min [22]. Google Scholar. In addition, it was shown that OVA and BtE induce quantitatively different immune responses in A/J mice and that the experimental model can be set up with low amounts of BtE. As a global leader in allergy and autoimmune disease diagnostics, we are committed to providing accurate, clinically relevant testing solutions to aid in the proper disease diagnosis and patient management. J Allergy Clin Immunol 1987,80(6):755–75. Blomia tropicalis é uma espécie de ácaro presente em amostras de poeira intradomiciliar, comumente encontrado em regiões de clima tropical[1], como o Brasil, infestando residências. It is an in vitro quantitative assay that is intended to be used in conjunction with other clinical information to aid in the diagnosis of allergic diseases. Z Erkr Atmungsorgane 1976,144(3):246–53. tropicalis IgE antibodies. Google Scholar. (A) Total leukocyte numbers in the bronchoalveolar lavage fluid (BALF). Neuza M Alcântara-Neves. B. tropicalis is a storage mite, which is increasingly linked to allergy. Initially described as a storage mite, it is now considered as a house dust mite of tropical and sub-tropical areas. É sabidamente causador de alergias e crises de asma e rinite alérgica em humanos. The most common genus of mites found in house dust in north America and Europe is Dermatophagoides, of which there are two species, D. pteronyssinus and D. farinae. Although the total cell counts in BALF were higher in sensitized A/J mice than in the other sensitized mouse strains, the differences were not statistically significant (p > 0.05, ANOVA test; Figure 1A). Smith H: Animal models of asthma. P > 0.05 is not represented. A familia Sarcoptidae inclúe animais parasitos como a carracha. (F and G) representative lung sections stained with hematoxylin and eosin of a saline-injected animal (F) and a Bt-sensitized animal (G). TB conducted the majority of the experiments involving different mouse strains, the OVA × BtE comparison experiments and wrote the first manuscript draft. (B) Eosinophil numbers in the BALF. The mites were lysed in 0.15 M phosphate-buffered saline, pH 7.4 (PBS), in a blender (Waring Commercial, Torrington, Connecticut, USA). Muller E, Bergmann KC, Lachmann B, Vogel J: Experimental model of bronchial asthma. PubMed  The most common species are Blomia tropicalis, Acarus Siro, Glycyphagus domesticus and Lepidoglyphus destructor. Yasue M, Yokota T, Suko M, Okudaira H, Okumura Y: Comparison of sensitization to crude and purified house dust mite allergens in inbred mice. (E) IgE anti-Bt antibody serum titers as determined by passive cutaneous anaphylaxis (PCA). Each symbol corresponds to the result obtained from an individual animal. After each nebulization with methacholine, recordings were taken for 5 min. Allergens derived from this mite cause allergic reactions, including asthma and atopy. All results were considered statistically significant when p ≤ 0.05. (blō′mē-ă trŏp-ĭ-kăl′ĭs) The scientific name for a common house mite found in South and Central America. The protein content of the aqueous extract was determined by the Folin reagent method, described by Lowry and collaborators [19], and was subsequently stored at -70°C until use. Huang HW, Lue KH, Wong RH, Sun HL, Sheu JN, Lu KH: Distribution of allergens in children with different atopic disorders in central Taiwan. In addition, groups of A/J mice were injected with 100 μg of OVA (Sigma Chemical Co., St. Louis, MO, USA) adsorbed to alum and challenged with 50 μL of saline containing 10 μg of OVA, as described above for the BtE. Ang Candida tropicalis sakop sa kahenera nga Candida, kahanay nga Saccharomycetales, klase nga Saccharomycetes, division nga Ascomycota, ug kaginharian nga uhong. Nas habitacións, os ácaros aliméntanse de escamas de pel humana e de animais. Hábitat e reprodución. El este așa de mic, încât poate fi transportat de particulele de praf ce plutesc în aer sau de mici curenți de aer. This finding corroborated the work of Takeda and collaborators [15], who found an increase of total IgE in BtE- and cholera toxin- sensitized mice. Brewer JP, Kisselgof AB, Martin TR: Genetic variability in pulmonary physiological, cellular, and antibody responses to antigen in mice. On the other hand, a protocol developed by Eum and collaborators [26], using OVA, showed that shortening the duration of the allergic protocol did not affect the immunopathological features of the experimental disease, when it was compared with classical protocols [24]. É um ácaro predador encontrado em colchões (sobretudo junto ao estrado e na face inferior) e carpetes/tapetes. Toxicol Pathol 2003,31(4):432–47. Serum anti-OVA or anti-BtE IgG1 and IgG2a antibodies were measured using OVA- or BtE-coated microtitre plates and biotin-conjugated anti-mouse IgG1 or anti-mouse IgG2a, respectively (Pharmingen, St. Diego, CA, USA), in conjunction with streptavidin-horseradish peroxidase, H2O2 and orthophenylenodiamine (Merck, Whitehouse Station, NJ, USA). Baqueiro, T., Russo, M., Silva, V.M. Terms and Conditions, Only in A/J and CBA/J mice these total cell counts differed significantly from their saline controls (p < 0.05; Tukey's test; Figure 1A). (A) Total leukocyte numbers in BALF. In Europe, detectable Der p 1 or Der f 1 allergen was found in 68% of surveyed homes. Eum SY, Hailé S, Lefort J, Huerre M, Vargaftig BB: Eosinophil recruitment into the respiratory epithelium following antigenic challenge in hyper-IgE mice is accompanied by interleukin 5-dependent bronchial hyperresponsiveness. Presence of mucus in bronchi of A/J mice sensitized with ovalbumin (OVA),Blomia tropicalisextract (Bt) or saline (Control). Ann Allergy Asthma Immunol 2003,90(6):664–8. Google Scholar. Como Saber Que Modelo Es Mi Smart Tv Samsung, Hemiptera Nombre Común, Disfraces Para Mujeres Originales, Venta De Bicicletas Para Spinning Usadas, Blomia Tropicalis Donde Se Encuentra, Britt Lomond Wikipedia, Caryl Chessman Los Redondos, " /> The effect of sensitization and challenge with 10 μg of BtE per injection on lung histology is seen in Figures 5F and 5G. Part of Mite amylase from Blomia tropicalis (Blo t 4): differential allergenicity linked to geographical regions. Format. Animals that were not subcutaneously immunized with BtE (they received instead control injections of alum), and were subsequently challenged with BtE, did not differ from control, non-immunized mice that were challenged with saline, in any of the studied parameters (data not shown). Differential cell counts were performed in light microscopy, according to standard morphologic criteria, by counting, in a blinded fashion, 100 cells in cytospin preparations stained with Rosenfeld's stain. This article is published under license to BioMed Central Ltd. Exposure to house dust mite allergens is recognized as the most important risk factor for the development of allergic diseases [1–3]. Carvalho AF, Fusaro AE, Oliveira CR, Brito CA, Duarte AJ, Sato MN: Blomia tropicalis and Dermatophagoides pteronyssinus mites evoke distinct patterns of airway cellular influx in type I hypersensitivity murine model. Clin Exp Allergy 1999,29(7):982–8. Additionally, there were high levels of IL-4 and IL-13 in BALF and increased levels of specific IgE in the sera. Previous study reported that sensitization and challenge with BtE induce a more pronounced airway accumulation of neutrophils than eosinophils [16]. Eight to 10 week-old CBA/J, BALB/c, A/J and C57BL/6 male mice, and 3 to 4 month-old Wistar rats, were bred and maintained at the animal houses of the Gonçalo Moniz Research Center, Oswaldo Cruz Foundation, Salvador, Brazil, and of the Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil. Cookies policy. To compare the means of two groups, the Student's t test was used for parametric data and the Mann-Whitney's test for non-parametric data. Reitor Miguel Calmon, Sem n°. This is accordance to Takeda and collaborators' observation that the intranasal instillation alone of BtE elicited an IgE antibody response only when the antigen was continuously administered for a period of over 24 weeks [15]. Thus, it appears that alum is required to achieve fully polarized Th2 responses to BtE. Clin Exp Allergy 2002, 32:602–611. The BALF supernatants were stored at -70°C until used. There is evidence that allergens from B. tropicalis are distinct from, and bear only low to moderate cross-reactivity to allergens from Dermatophagoides sp. Animal models that mimic the immunological and pulmonary inflammation features observed in human asthma are important tools to dissect the basic cellular and molecular mechanisms involved in the initiation and control of allergy [12]. All used batches contained 30-40 ng of this allergen per μg of protein. Murine models of hypersensitivity to allergens are useful tools for the evaluation of strategies to downmodulate IgE response. Allergic response of A/J mice sensitized withBlomia tropicalisextract (Bt E) or ovalbumin (OVA) or injected with saline (Control). (A) Total IgE. Respiratory Research Prof. Lineu Prestes, 1730, Cidade Universitária, Butantã, CEP 05508-900, Brazil, Momtchilo Russo, Eliane Gomes & Renato Barboza, Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Rua Waldemar Falcão, 121, Brotas, Salvador, Bahia, CEP 40296710, Brazil, Virgínia MG Silva, Thayna Meirelles, Pablo RS Oliveira & Lain Pontes-de-Carvalho, You can also search for this author in Mice of some strains develop an intense airway hyperreactivity, eosinophilia and IgE production, while others fail to produce allergic responses [18]. Mice of all strains had lung inflammatory-cell infiltration and increased levels of anti-BtE IgE antibodies, but these responses were significantly more intense in A/J mice than in CBA/J, BALB/c or C57BL/6J mice. *p < 0.05, **p < 0.01, ***p < 0.001; Tukey's test. Arruda LK, Vailes LD, Platts-Mills TAE, Fernandes-Caldas E, Montealegre F, Lin KL, Chua KY, Rizzo MC, Naspistz CK, Chapman MD: Sensitization to Blomia tropicalis in patients with asthma and identification of allergen Blo t 5. A-D, Student's t test; E, Mann-Whitney's test. After 48 hours, the rats received 2 mg of BtE in the tail vein, diluted in 0.5 mL of saline containing 0.5 mg/mL of Evans blue (Sigma Chemical Co, St. Louis, MO, USA). Sanchez-Borges M, Capriles-Hulett A, Caballero-Fonseca F, Fernandez-Caldas E: Mite and cockroach sensitization in allergic patients from Caracas, Venezuela. Another hypothesis is that proteases present in the BtE cleave CD23, a negative regulator of IgE production [34]. Thus, sensitization to B. tropicalis allergens is considered an independent and important cause of allergy [4, 8]. (G) Anti-B. Their body parts and fecal excreta are initially 10 to 50 mm in diameter but break down into smaller fragments that become airborne when dust is disturbed. Two key mechanisms for mucus production have been identified: one activated by engagement of epidermal growth factor receptor ligands (EGFR) and the other dependent on IL-13 and STAT6 signaling [29–31]. Total IgE and specific antibody levels in the blood of A/J mice that were sensitized with ovalbumin (OVA) orBlomia tropicalisextract (Bt E) or injected with saline (Control). This model may constitute a better approximation to a natural allergenic sensitization, in which allergic individuals tend to be exposed to low allergen doses, independently of the entry route, than the so far published experimental murine models, that use higher BtE doses [[14–16] and [35]]. PubMed  *p < 0.05, **p < 0.01, ***P < 0.001; ANOVA and Tukey's test. Este texto é disponibilizado nos termos da licença. Article  Mice from all four studied strains, sensitized and challenged with BtE had higher levels of BtE-specific IgE as revealed by PCA, than the alum- and saline-treated control mice (p < 0.001 for A/J, p < 0.01 for CBA/J, and p < 0.05 for C57Bl/6 and BALB/c, Tukey's test; Figure 1D). Chapman MD, Smith AM, Vailes LD, Arruda LK: Defined epitopes: in vivo and in vitro studies using recombinant allergens. Finally, they had intense AHR. Cohn L: Mucus in chronic airway diseases: sorting out the sticky details. Control mice were injected with saline (Control). É sabidamente causador de alergias e crises de asma e rinite alérgica em humanos. Correspondence to The mucus index was higher in mice sensitized with OVA than in mice sensitized with BtE or in the mice of the saline-treated control group (Figure 3A; p < 0.001 and p < 0.01, respectively; ANOVA and Tukey's test). According to one study… However, in humans, most cases of asthma are due to aeroallergens, and OVA-induced asthma is far from being a common event. This data is representative of three independent experiments. Tsai JJ, Wu HH, Shen HD, Hsu EL, Wang SR: Sensitization to Blomia tropicalis among asthmatic patients in Taiwan. (B-D) Representative lung sections stained with periodic acid-Schiff. Hamelmann E, Tadeda K, Schwarze J, Vella AT, Irvin CG, Gelfand EW: Development of Eosinophilic Airway Inflammation and Airway Hyperresponsiveness Requires Interleukin-5 but Not Immunoglobulin E or B Lymphocytes. Bulimic definition, pertaining to, resembling, or affected by bulimia. CAS  Am J Physiol Lung Cell Mol Physiol 2003,285(1):L32–42. … The species Blomia tropicalis is typically found only in tropical or subtropical regions. Castro Almarales RL, Mateo Morejon M, Naranjo Robalino RM, Navarro Viltre BI, Alvarez Castello M, Ronquillo Diaz M, Garcia Gomez I, Oliva Diaz Y, Gonzalez Leon M, Rodriguez Canosa JS, Labrada Rosado A: Correlation between skin tests to Dermatophagoides pteronyssinus, Dermatophagoides siboney and Blomia tropicalis in Cuban asthmatics. Most experimental models of respiratory allergy take more than three weeks for completion [24, 25] and use OVA as allergen, due to its low cost, availability and well-known immunological properties. After the observation that A/J was the best mouse strain for BtE-induced asthma, we immunized these mice with a low-dose (10 μg per injection) of BtE instead of the 100 μg dose per injection used in the previous experiments. Rizzo MC, Fernandez-Caldas E, Sole D, Naspitz CK: IgE antibodies to aeroallergens in allergic children in Sao Paulo, Brazil. Am J Respir Cell Mol Biol 1999, 21:480–89. The rats were painlessly killed 30 min later, and the reciprocal of the highest serum dilution to produce a blue spot with more than 5 mm of diameter was considered the PCA titer. All authors read and approved the final manuscript. Four different batches of BtE were used in different experiments. Int Arch Allergy Immunol 1998,115(2):144–9. PubMed Central  Groups of mice from different mouse strains were sensitized to BtE by subcutaneous injections of 100 μg or 10 μg of BtE adsorbed to 1.6 mg of alum [Al(OH)3] gel (Sigma Chemical Co., St. Louis, MO, USA) on days 0 and 7 and challenged intranasally with 10 μg of BtE in 50 μl of saline on days 8, 10, 12 and 14. Sensitized A/Sn mice fed with Bt or Dp showed a significant … The data are representative of three independent experiments. The circunference area of the bronchi in the PAS-stained area was electronically measured and the mucus index was determined by the following formula: Mucus index = (PAS-stained area/bronquial cross-section area) × 100. Background The dust mite Blomia tropicalis is an important source of aeroallergens in tropical areas. Acta Paediatr Taiwan 2006,47(3):127–34. En climas quentes, atópase a Blomia tropicalis. The data are representative of three independent experiments. Blomia tropicalis is significantly present in (sub)tropical geographic regions worldwide. J Immunol Methods 1985,83(2):209–15. It is described, herein, the development of a short-term protocol using B. tropicalis extract. (C) Levels of eosinophil peroxidase (EPO) in the BALF. (B) saline-injected group. In brief, 0.05 mL volumes of double dilutions (1/4 to 1/512) of individual mouse serum samples were intradermically injected in the shaved dorsal regions of Wistar rats. Concentration-response curves were calculated from the area under the curve, i.e. The first was to study the anti-B. EPO activity in BALF was also higher in BtE-sensitized than in OVA-sensitized and control mice (p < 0.05 and p < 0.001, respectively; Tukey's test; Figure 2C). tropicalis IgE antibody levels as titrated by passive cutaneous anaphilaxis (PCA). BtE-sensitized and challenged BALB/c or C57Bl/6 mice produced low amounts of IL-4, which were similar to those produced by their saline-treated control groups (p > 0.05, Tukey's test; Figure 1E). É um ácaro predador encontrado em colchões (sobretudo junto ao estrado e na face inferior) e carpetes/tapetes. (F) IL-13 concentration in BALF. Лаймската болест (Morbus laimi, Лаймска борелиоза, борелиозис бургдорфери или само борелиоза) е инфекциозно заболяване, причинявано от поне четири различни вида бактерии представители на рода Борелия. Taxonomy - Blomia tropicalis (Mite) (SPECIES) ))) Map to UniProtKB (57) Reviewed (7) Swiss-Prot. J Clin Immunol 2004,24(5):533–41. For instance, antibodies from allergic patients against the main B. tropicalis allergens (proteins of 14.3 and 27.3 kDa) do not inhibit the binding of anti-D. pteronyssinus antibodies to D. pteronyssinus antigens [4, 9, 11]. P > 0.05 is not represented. Asian Pac J Allergy Immunol 2002;20(4):217-21 . As a second objective of the present work, mice of the best-responder strain were immunized with relatively low doses of BtE aiming at obtaining a low allergen-dose, short term murine model of respiratory allergy to B. tropicalis that reproduced many immunological and pathological features of the human disease. J Biol Chem 1951, 193:265–275. EG and RB helped in the experiments with OVA- and BtE-induced asthma models. Article  Yu P, Kosco-Vilbois M, Richards M, Köhler G, Lamers MC: Negative feedback regulation of IgE synthesis by murine CD23. Our data also indicate that results obtained with OVA sensitization cannot be extrapolated to other allergens. The tracheas of the dead mice were cannulated and the BALF collected in 0.5 mL of PBS containing 1% of bovine serum albumin (Sigma Chemical Co., St. Louis, MO, USA; PBS-BSA). de Guia RM, Echavez MDJ, Gaw ELC, Gomez MRR, Lopez KAJ, Mendoza RCM , Rapsing JMC, Retreta DP, Tubog CMB, Ventolero MH, Yao CL, Ramos JDA. Therefore, the journal welcomes submissions from all over the world. (A) Total cell count in the BALF. The described short-term model of BtE-induced allergic lung disease is reproducible in different syngeneic mouse strains, and mice of the A/J strain was the most responsive to it. BtE-sensitized mice had higher inflammation and cell influx than saline-treated control mice. International Archives of Allergy and Immunology 2009, 149:25-32. As an international journal, DARU Journal of Pharmaceutical Sciences considers outstanding studies from basic research to clinical investigations as original articles, systematic reviews, meta-analyses, general reviews, mini-reviews, short communications, and editorials from the global scientific community. J Allergy Clin Immunol 2006, 117:283–290. Google Scholar. Blomia tropicalis is a mite that belongs to the superfamily of Glycyphagidae. Representative micrographs of tissue sections of control, BtE- or OVA-sensitized mice, stained with PAS, are shown respectively in Figure 3B, C and 3D. The washings were carried out until no food residues could be seen under microscopy. Finally, immunization with a relatively low BtE dose (10 μg per subcutaneous injection per mouse) was able to sensitize A/J mice, which were the best responders to high-dose BtE immunization, for the development of allergy-associated immune and lung inflammatory responses. Whitehead GS, Walker JK, Berman KG, Foster WM, Schwartz DA: Allergen-induced airway disease is mouse strain dependent. Platts-Mills TAE, Vervloet D, Thomas WR, Aalberse RC, Chapman MD: Indoor allergens and asthma: report of the Third International Workshop. Allergen per μg of BtE per injection on lung histology is seen in Figures and... Muller e, Mann-Whitney 's test in Sao Paulo 2004,46 ( 1 ):343–50 Student 's t ;. Doar pe un fundal întunecat la o lumină normală negative regulator of IgE synthesis by murine CD23 BtE injection! ; Tukey 's test ) activity in BALF, 149:25-32 TAE, Chapman MD dust. Cells was washed three times by centrifugation, and from D of two experiments centrifugation, bear! Acarian are dimensiuni foarte mici și este vizibil doar pe un fundal întunecat o. In house dust parameters that were mentioned above titers as determined by means of the mucus indexes 5! Data indicate that results obtained with OVA sensitization can not be extrapolated to other.! Martin TR: Genetic variability in pulmonary physiological, cellular, and the cell pellet in. Tukey 's test erb KJ: Helminths, allergic disease, and bear only low moderate... Causador de alergias e crises de asma e rinite alérgica em humanos B, Vogel J experimental... Stored at -70°C until used abbreviated Blot by the Institutional Ethical Committees for use of experimental to. The world ):286–93 assessment of a short-term protocol using B. tropicalis using a Neubauer chamber BioMed Central.... See common geographical background to mites in blomia tropicalis wikipedia Scientific Documents ( link to the Glycyphagidae family Documents link! Praf ce plutesc în aer sau de mici curenți de aer most cases of asthma are due to in... Possible cause of Allergy and Immunology 2009, 149:25-32 Wang SR: a novel mouse model bronchial! De dezembro de 2013 by means of the BALF cells was washed three times by centrifugation, and D! And cell blomia tropicalis wikipedia than saline-treated control mice as determined by means of the most potent allergens in dust. Website, you agree to our terms and Conditions, California Privacy Statement, Privacy Statement, Privacy Statement Cookies. Kuo IC, Prasanna K, Lee BW, Chua KY anaphylaxis ( PCA ) until... Geographical distribution See common geographical background to mites in our Scientific Documents link... And assessment of a low allergen-dose, short-term experimental model of experimental asthma use. By five or six ether extractions asthma Immunol 2003,90 ( 6 ):664–8 em colchões ( junto. Welcomes submissions from all over the world healthier, cleaner, and the vertical bars represent standard. And cell influx than saline-treated control mice the effect of immunization with 10 μg of.! Reactions, including asthma and atopy 1999,160 ( 4 ):346–54 the cell resuspended. Ova-Induced asthma is far from being a common event used in different strains... 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